yr12student22
Just to add to this, the lac operon is no longer in the bio study design, as it has been replaced with the trp operon. However, they both do share similar principles.
What does the trp operon demonstrate?
It demonstrates the importance of gene expression regulation to aid in the conservation of energy and biochemical resources.
What does it require?
Not sure what you mean, but it involves the amino acid tryptophan. It also involves RNA polymerase, repressor proteins, ribosomes, and tRNA.
What does it produce?
When the structural genes are transcribed and translated, they form enzymes that convert different chemicals into tryptophan. This is what is specifically produced (don't need to know, but it can be helpful when understanding how it all works):
• TrpE and TrpD -> Form enzyme Anthranilate synthase, which converts chorismate (found in the cell) into anthranilate.
• TrpC -> Forms enzyme Indole-3-glycerol-photosphate synthase, which converts anthranilate into indole-3-glycerol-photosphate.
• TrpB and TrpA -> Forms enzyme Tryptophan synthase, which converts indole-3-glycerol-photosphate into tryptophan.
(Requires lots of energy, meaning the process needs to be tightly regulated)
Here is the trp operon order as well to make the above answer make more sense haha.
3' end - Promoter - Operator - Leader (TrpL) - Attenuator - TrpE - TrpD - TrpC - TrpB - TrpA - Trailer - 5' end
You do need to know about the two methods of gene expression regulation, which are repression and attenuation (I think the lac operon only involved a repression mechanism, but the trp operon involves the process of attenuation as well, which is more complex).
